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Place the Following Steps in Cloning DNA in the Correct

Question 39

Multiple Choice

Place the following steps in cloning DNA in the correct order
1 - use vector to deliver new rDNA to bacterial or other cells
2 - use restriction enzymes to cut a plasmid and add the desired gene
3 - isolate and cut out a desired gene using restriction enzymes
4 - use DNA ligase to seal the new gene
5 - allow bacterial cells to replicate and produce desired product


A) 1, 2, 3, 4, 5
B) 5, 3, 2, 4, 1
C) 2, 5, 3, 1, 4
D) 3, 2, 4, 1, 5

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