In an Attempt to Monitor the Movement of Individual Kinesin

Multiple Choice

In an attempt to monitor the movement of individual kinesin molecules labeled with GFP along microtubules labeled with a red fluorescent dye using an in vitro motility assay,what specialized type of laser-based fluorescence microscopy would be most likely to be employed?

A) FRAP
B) TIRF
C) total internal reflection microscopy
D) atomic force microscopy
E) TIRF and total internal reflection microscopy