A Microscope Is Equipped with a Laser That Can Be

Multiple Choice

A microscope is equipped with a laser that can be focused on a small region of the cell.The laser beam is used to bleach fluorescent tubulin in a small region of the cell.The specimen is then followed over time and the recovery of the fluorescent signal into the bleached zone is then measured.What is the name of this technique?

A) TIRF
B) fluorescence recovery after photobleaching
C) total internal refraction microscopy
D) FRAP
E) fluorescence recovery after photobleaching and FRAP