What Technique Can Clarify the Dynamic Events That Occur as a Protein

Multiple Choice

What technique can clarify the dynamic events that occur as a protein functions by introducing into the protein chemical groups (called nitroxides) that contain an unpaired electron? The properties of these nitroxides are sensitive to the distance that separates them and the unpaired electron produces a characteristic spectrum that can be monitored by the technique in question.The technique yields information about the distance between selected protein residues.

A) site-directed mutagenesis
B) site-directed amenuensis
C) hydropathy plots
D) electron paramagnetic resonance (EPR) spectroscopy
E) infrared spectroscopy