Multiple Choice
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I.Transform bacteria with a recombinant DNA molecule.
II.Cut the plasmid DNA using restriction enzymes (endonucleases) .
III.Extract plasmid DNA from bacterial cells.
IV.Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V.Use ligase to seal plasmid DNA to non-plasmid DNA.
A) II, III, V, IV, I
B) III, II, IV, V, I
C) III, IV, V, I, II
D) IV, V, I, II, III
Correct Answer:

Verified
Correct Answer:
Verified
Q1: Transgenic mice are useful to human researchers
Q2: Suppose that a researcher is successful at
Q4: Why is sequencing an entire genome, such
Q6: Use the following information to answer the
Q8: In 1997, Dolly the sheep was cloned.
Q10: For a particular microarray assay (DNA chip),
Q11: Why might using retroviral vectors for gene
Q15: Which of the following processes helps bacterial
Q48: Which of the following enzymes is required
Q55: Which of the following processes uses labeled