In Shotgun Sequencing, How Is a Complete Sequence of a Genome

Multiple Choice

In shotgun sequencing, how is a complete sequence of a genome reconstructed?

A) The entire genome is directly sequenced chromosome by chromosome.
B) The genome is fragmented and sequences of each fragment are arranged so that overlapping regions are identified.
C) The sequences of a large number of individuals is obtained and "averaged" to obtain the complete sequence.
D) The position of each marker and gene are first mapped relative to regions for which the genomic sequence is not known.