You Are Using CRISPR-Cas Technology to Introduce a Single Nucleotide

Multiple Choice

You are using CRISPR-Cas technology to introduce a single nucleotide change in a gene of interest in living cells. You are designing your experiment. In which component of the reaction will you engineer the single nucleotide change?

A) Donor DNA
B) Spacer RNA
C) Repeat RNA
D) Linker RNA
E) tracrRNA