You Cloned a 2-Kb Fragment of DNA into a Bacterial

Multiple Choice

You cloned a 2-kb fragment of DNA into a bacterial cloning vector and want to construct a restriction map of the insert.You amplify the 2-kb insert using PCR, purify it, and subject it to differential digestion with the enzymes EcoRI and HindIII, gel-fractionate the digests, and visualize the restriction patterns by staining the gels with ethidium bromide to generate the following results.Using these data, indicate the CORRECT order of restriction sites in the DNA insert.

A) EcoRI, HindIII, EcoRI, HindIII, EcoRI
B) HindIII, EcoRI, HindIII, EcoRI, HindIII
C) HindIII, EcoRI, HindIII, HindIII, EcoRI
D) HindIII, EcoRI, EcoRI, HindIII, EcoRI
E) EcoRI, EcoRI, HindIII, EcoRI, HindIII