In Using a Cloning Vector with a Multiple Cloning Site

Multiple Choice

In using a cloning vector with a multiple cloning site spliced into the front part of the lacZ gene, production of beta-galactosidase, as shown by the blue color when grown on Xgal, indicates that:

A) an insert has been placed into the multiple cloning site.
B) the cells are antibiotic sensitive.
C) the cells lack plasmids.
D) the cells have not been successfully transformed.
E) there is no insert in the multiple cloning site.