You Identify an RFLP in Mice by Digesting Genomic DNA

Multiple Choice

You identify an RFLP in mice by digesting genomic DNA with HindIII enzyme and radiolabeling a piece of probe DNA.Southern blot analysis shows that the probe detects a 2-kb fragment in one strain of mice and a 4-kb fragment in another strain of mice.The two mice strains are crossed to produce an F₁ generation.Two F₁ siblings are mated to produce a dozen F₂ progeny.You isolate genomic DNA from several F₁ and F₂ individuals, digest with HindIII, and perform a Southern blot with the same probe. How many bands would be seen in the Southern blot of an F₂ individual?

A) 1
B) 2
C) 3
D) 4
E) Two of the answers are possible.