In Producing a Recombinant Plasmid to Be Used to Clone

Multiple Choice

In producing a recombinant plasmid to be used to clone a given donor insert, it is possible to cut both the donor and plasmid with the same restriction enzyme, resulting in complementary sticky ends. Assuming plenty of plasmid DNA is available, why is further selection necessary before the introduction of the plasmid into a cellular system?

A) Contamination will have introduced other donor inserts.
B) Some donor inserts will be single stranded and deteriorate.
C) Some donor inserts will be sensitive to particular antibiotics.
D) Some donor pieces will remain uninserted.
E) Some donor strands will be inserted with an incorrect orientation.