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What Is the Role of Cis-Acting and Trans-Acting Factors in Transcription

Question 19

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What is the role of cis-acting and trans-acting factors in transcription? Explain how these factors play a role in the lac operon, and discuss how complementation can be used to identify where the mutations have occurred (structural genes versus regulatory regions). Which type of mutations
can be complemented by a second gene with normal function? Are IS and OC mutants cis- or
trans-acting?

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If a factor is trans-acting, genes that ...

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