How Might a Single Base Insertion into the Second Codon

Multiple Choice

How might a single base insertion into the second codon of the coding sequence of a gene affect the amino acid sequence of a protein encoded by the gene?

A) A single amino acid could change.
B) The amino acid sequence would be altered.
C) The mutation may have no effect on amino acid sequence.
D) A single extra amino acid would be present in the protein.
E) All of the above are possible outcomes.