To Map Genes of a Bacterial Strain, Conjugation Must Be

Multiple Choice

To map genes of a bacterial strain, conjugation must be interrupted at given times. Suppose you have Hfr cells of genotype a⁺b⁺c⁺d⁺e⁺str^R and F^- cells of genotype a^-b^-c^-d^-e^-str^S and you combine these two cultures in liquid medium in four blenders at time 0. After intervals of 3, 6, 9, and 12 minutes, you turn on successive blenders. This graph shows the results of plating the cultures of each blender. What is the correct order of the genes on this chromosome?

A) a b c d e
B) a c e b d
C) b e c a (d is unknown)
D) d a c e b
E) b e c a d