In DNA Sequencing by the Sanger (Dideoxy) Method

Multiple Choice

In DNA sequencing by the Sanger (dideoxy) method:

A) radioactive dideoxy ATP is included in each of four reaction mixtures before enzymatic synthesis of complementary strands.
B) specific enzymes are used to cut the newly synthesized DNA into small pieces, which are then separated by electrophoresis.
C) the dideoxynucleotides must be present at high levels to obtain long stretches of DNA sequence.
D) the role of the dideoxy CTP is to occasionally terminate enzymatic synthesis of DNA where Gs occur in the template strands.
E) the template DNA strand is radioactive.