An Enzyme Can Catalyze a Reaction with Either of Two

Short Answer

An enzyme can catalyze a reaction with either of two substrates, S₁ or S₂. The K_m for S₁ was found to be 2.0 mM, and the K_m, for S₂ was found to be 20 mM. A student determined that the V_max was the same for the two substrates. Unfortunately, he lost the page of his notebook and needed to know the value of V_max. He carried out two reactions: one with 0.1 mM S₁, the other with 0.1 mM S₂. Unfortunately, he forgot to label which reaction tube contained which substrate. Determine the value of V_max from the results he obtained:
Tube number
Rate of formation of product
1
0.5
2
4.8
3
4
5