You Measure the Initial Rate of an Enzyme Reaction as a Function

Essay

You measure the initial rate of an enzyme reaction as a function of substrate concentration in the presence and absence of an inhibitor. The following data are obtained:
[S]
V₀
-Inhibitor
+Inhibitor
0.0001
33
17
0.0002
50
29
0.0005
71
50
0.001
83
67
0.002
91
80
0.005
96
91
0.01
98
95
0.02
99
98
0.05
100
99
0.1
100
100
0.2
100
100
a) What is the V_max in the absence of inhibitor?
b) What is the K_m in the absence of inhibitor?
c) When [S] = 0.0004, what will V₀ be in the absence of inhibitor?
d) When [S] = 0.0004, what will V₀ be in the presence of inhibitor?
e) What kind of inhibitor is it likely to be?