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The Image Below Plots the Denaturation of Ribonuclease a as a Function

Question 58

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The image below plots the denaturation of ribonuclease A as a function of guanidine hydrochloride (GdnHCl) concentration, monitored by circular dichroism. The shape of the curve in the figure supports which statement? The image below plots the denaturation of ribonuclease A as a function of guanidine hydrochloride (GdnHCl)  concentration, monitored by circular dichroism. The shape of the curve in the figure supports which statement?   A)  The addition of GdnHCl has little effect on protein secondary structure. B)  Increasing temperature causes the protein to become unfolded. C)  Unfolding of this protein is a cooperative process. D)  Ribonuclease A is a heat-stable protein. E)  The peptide bonds of the protein are broken around 3.4 M GdnHCl.


A) The addition of GdnHCl has little effect on protein secondary structure.
B) Increasing temperature causes the protein to become unfolded.
C) Unfolding of this protein is a cooperative process.
D) Ribonuclease A is a heat-stable protein.
E) The peptide bonds of the protein are broken around 3.4 M GdnHCl.

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