To Determine the Isoelectric Point of a Protein, First Establish

Multiple Choice

To determine the isoelectric point of a protein, first establish that a gel:

A) contains a denaturing detergent that can distribute uniform negative charges over the protein's surface.
B) exhibits a stable pH gradient when ampholytes become distributed in an electric field.
C) is washed with an antibody specific to the protein of interest.
D) neutralizes all ionic groups on a protein by titrating them with strong bases.
E) relates the unknown protein to a series of protein markers with known molecular weights, M_r.