Which Effect Is NOT Expected from Changing the Sequence in the -10

Multiple Choice

Which effect is NOT expected from changing the sequence in the -10 region of an E. coli promoter for a protein-coding gene?

A) decreased binding of $\sigma$ subunits
B) decreased transcription of the mRNA
C) decreased binding of ribosomes to the mRNA
D) lower concentrations of the protein product in the cell
E) All of these effects would be expected.