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What Acts as the Nucleophile in the Mechanism of Nucleotide

Question 39

Multiple Choice

What acts as the nucleophile in the mechanism of nucleotide addition by RNA polymerase?


A) the 5' phosphate of an incoming nucleotide
B) a water molecule
C) a 5' hydroxyl of the template DNA
D) a 3' hydroxyl from the RNA being extended
E) an aspartate in the active site

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