Which Statement Does NOT Apply to the Topoisomerase IA Mechanism

Multiple Choice

Which statement does NOT apply to the topoisomerase IA mechanism?

A) A covalent bond is formed from the protein to the 3' phosphate of a nucleotide.
B) Two strands of the nucleic acid remains intact.
C) The action of the enzyme changes the linking number by single increments.
D) The action of the enzyme will decrease the magnitude of the superhelical density.
E) Energy for the process comes from the supercoiling strain in the nucleic acid.