Which Process Is NOT Involved in the Steps of Glycolysis

Multiple Choice

Which process is NOT involved in the steps of glycolysis between glyceraldehyde 3-phosphate and 3-phosphoglycerate?

A) a substrate-level phosphorylation reaction
B) catalysis by an isomerase enzyme
C) reduction of NAD⁺ to NADH
D) the formation of a mixed anhydride intermediate.
E) the indirect coupling of reactions catalyzed by a kinase and a dehydrogenase enzyme