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A Molecular Biologist Carries Out a PCR Reaction and Discovers

Question 123

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A molecular biologist carries out a PCR reaction and discovers to his disappointment that no PCR amplification takes place. It turns out that the problem is one of the primers, which has the following sequence:

5'-ATAACACTAAATAGCGCTATTTAGTGTTAT-3'

Can you suggest a reason why this primer does not work for PCR?

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The last 15 nucleotides of the primer ar...

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