You Are Interested in Cloning a Fragment from the Genome

Multiple Choice

You are interested in cloning a fragment from the genome of an organism where the G+C content of the sequence is 50%. The gene you wish to study is 2 kb (kilobase, each kb is 1000 bases) in length and does not include cleavage sites for any of the restriction enzymes below. For convenience in later experimental procedures, you want the average size of the fragments produced by the restriction enzyme to be approximately 4 kb. Which of the following restriction enzyme(s) would you choose to digest your genomic DNA?

A) AluI
B) EcoRI
C) NotI
D) Swa-I