A Graduate Student Wants to Create a Recombinant DNA Molecule

Multiple Choice

A graduate student wants to create a recombinant DNA molecule and introduce this molecule into bacteria. What is the CORRECT order of steps that he should follow?

A) choose plasmid/donor DNA  cut with restriction enzymes  join fragments via DNA ligase  transform bacteria
B) choose plasmid  cut with restriction enzymes  join fragments with DNA ligase  introduce donor DNA  transform bacteria
C) join plasmid and donor DNA using DNA ligase  cut with restriction enzymes  transform bacteria
D) transform bacteria  choose plasmid/donor DNA  join fragments with DNA ligase
E) cut plasmid/donor DNA with restriction enzymes  transform bacteria  isolate donor DNA/plasmid  join fragments with DNA ligase