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Piezo1 and Piezo2 Were Identified in an RNAi Knockdown Screen

Question 19

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Piezo1 and Piezo2 were identified in an RNAi knockdown screen in a mouse neuroblastoma cell line. State whether each of the following experiments suggested that at least one Piezo was necessary, sufficient, or present in the right time and place to mediate touch mechanotransduction in vivo.

Premises:
Piezo2 (but not Piezo1) was expressed in a subset of DRG neurons.
Misexpression of Piezo1 or Piezo2 in cells conferred mechanically induced inward current.
Conditional knockout of Piezo2 in Merkel cells and adult sensory neurons abolished behavioral responses to low-force mechanical stimuli.
Purified Piezo1 formed an ion channel in artificial lipid bilayers.
Responses:
present in right time and place
sufficient
necessary

Correct Answer:

Piezo2 (but not Piezo1) was expressed in a subset of DRG neurons.
Misexpression of Piezo1 or Piezo2 in cells conferred mechanically induced inward current.
Conditional knockout of Piezo2 in Merkel cells and adult sensory neurons abolished behavioral responses to low-force mechanical stimuli.
Purified Piezo1 formed an ion channel in artificial lipid bilayers.
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