Deck 9: Agglutination

Question 1

When carrier particles are coated with an antigen that is not normally found on them,this is known as:

A) direct agglutination
B) passive agglutination
C) reverse passive agglutination
D) hemagglutination

Answer 1

passive agglutination

Question 2

Which antibody class may require the use of an enhancement technique to visualize the reaction?

A) IgM
B) IgG
C) IgA
D) IgE

Answer 2

IgG

Question 3

What would be the best test to determine evidence of hemolytic disease of the newborn?

A) direct antiglobulin testing
B) passive agglutination
C) coagglutination testing
D) indirect antiglobulin testing

Answer 3

indirect antiglobulin testing

Question 4

A serological reaction is set up in which the antigen is bound to a large carrier,the antibody is soluble,and they bind and form an insoluble complex that is detected macroscopically.What type of assay is described?

A) precipitation
B) agglutination
C) flocculation
D) neutralization

Question 5

Coombs' reagent is used for what purpose?

A) to link small antigens on patient red cells
B) to enhance agglutination with IgG coated red blood cells
C) to enhance agglutination with IgM coated red blood cells
D) to cause a precipitation reaction with small amounts of antibody

Question 6

Which of the following would enhance an agglutination reaction?

A) polyethylene glycol (PEG)
B) high ionic strength saline
C) running the reaction at 4C
D) running the reaction at a high pH

Question 7

If a Coombs' test is positive,which of the following is true?

A) Antibody has coated patient red blood cells in vitro.
B) Antigen is reacting with patient antibody.
C) Antibody has coated patient red blood cells in vivo.
D) Antigen has coated patient red blood cells in vivo.

Question 8

The process by which particulate antigens such as cells aggregate to form larger complexes when specific antibody is present is called:

A) sensitization
B) precipitation
C) agglutination
D) flocculation

Question 9

When a patient's red blood cells combine with anti-A typing serum to produce a positive result,this reaction is known as:

A) passive agglutination
B) reverse passive agglutination
C) hemagglutination
D) flocculation

Question 10

A serological test that uses red blood cells coated with exogenous antigens such as bacterial polysaccharides as a method to detect patient antibodies against that exogenous antigen is called:

A) latex agglutination
B) hemagglutination
C) neutralization
D) complement fixation

Question 11

In performing blood typing using the tube method,if the red cell button is not resuspended properly,what are the most likely results?

A) false-positive
B) false-negative
C) doesn't affect the results
D) makes the reaction weaker