Question 1

What is the purpose of adding antibody-sensitized red cells following the antiglobulin test?

Accepted Answer

A)  Ensure a weak antibody reaction was not missed 
B)  Confirm positive reactions 
C)  Check that the wash procedure was sufficient to remove unbound antibodies 
D)  Check that sufficient incubation took place 
A)  Ensure a weak antibody reaction was not missed 
B)  Confirm positive reactions 
C)  Check that the wash procedure was sufficient to remove unbound antibodies 
D)  Check that sufficient incubation took place C

Question 2

MATCHING
Select the reagent from the list below and match it to the routine blood banking procedure.
-Reagent used in the identification of ABO antibodies

Accepted Answer

A) Panel cells 
B) Screening cells 
C) A1 and B cells 
D) ABO antisera 
E) Lectins 
A) Panel cells 
B) Screening cells 
C) A1 and B cells 
D) ABO antisera 
E) Lectins C

Question 3

Rouleaux is a false-positive reaction caused by elevated serum protein levels.Which of the following tests would not likely be affected by an elevated protein level?

Accepted Answer

A)  Immediate-spin antibody screen 
B)  Direct antiglobulin test 
C)  Reverse typing in the ABO test 
D)  ABO forward typing 
A)  Immediate-spin antibody screen 
B)  Direct antiglobulin test 
C)  Reverse typing in the ABO test 
D)  ABO forward typing B

Question 4

Which of the following statements is true regarding IgG-sensitized red cells?

Accepted Answer

A)  They must be used to confirm a negative antiglobulin tube test. 
B)  They must be used to confirm a positive antiglobulin test. 
C)  They must be used to confirm a direct antiglobulin test that was negative with anti-C3d. 
D)  They should be used only with the indirect antiglobulin test. 
A)  They must be used to confirm a negative antiglobulin tube test. 
B)  They must be used to confirm a positive antiglobulin test. 
C)  They must be used to confirm a direct antiglobulin test that was negative with anti-C3d. 
D)  They should be used only with the indirect antiglobulin test.

Question 5

Why is polyethylene glycol reagent added to the screen or panel?

Accepted Answer

A)  Enhance detection of IgM antibodies. 
B)  Eliminate the reactivity of certain antigens. 
C)  Increase the avidity of IgG antibodies. 
D)  Eliminate the need for washing in the indirect antiglobulin test. 
A)  Enhance detection of IgM antibodies. 
B)  Eliminate the reactivity of certain antigens. 
C)  Increase the avidity of IgG antibodies. 
D)  Eliminate the need for washing in the indirect antiglobulin test.

Question 6

Select the antiglobulin test that best fits the descriptions below.A selection may be used more than once.
-Tests for certain clinical conditions such as hemolytic disease of the newborn and autoimmune hemolytic anemia

Accepted Answer

A) Indirect antiglobulin test 
B) Direct antiglobulin test 
C) Both the direct and indirect antiglobulin test 
A) Indirect antiglobulin test 
B) Direct antiglobulin test 
C) Both the direct and indirect antiglobulin test

Question 7

Select the antiglobulin test that best fits the descriptions below.A selection may be used more than once.
-Incubation step is not necessary

Accepted Answer

A) Indirect antiglobulin test 
B) Direct antiglobulin test 
C) Both the direct and indirect antiglobulin test 
A) Indirect antiglobulin test 
B) Direct antiglobulin test 
C) Both the direct and indirect antiglobulin test

Question 8

Following centrifugation of the gel card,red cells are evenly dispersed throughout one of the microtubes.This reaction could be graded as a:

Accepted Answer

A)  4+. 
B)  3+. 
C)  2+. 
D)  1+. 
A)  4+. 
B)  3+. 
C)  2+. 
D)  1+.

Question 9

What specificities does polyspecific antihuman globulin contain?

Accepted Answer

A)  Anti-IgG. 
B)  Anti-C3b and anti-C3d. 
C)  Anti-IgG and anti-C3d. 
D)  Anti-IgG and anti-IgM. 
A)  Anti-IgG. 
B)  Anti-C3b and anti-C3d. 
C)  Anti-IgG and anti-C3d. 
D)  Anti-IgG and anti-IgM.

Question 10

What reagent contains antibodies to multiple antigenic epitopes?

Accepted Answer

A)  Polyclonal-based 
B)  Monoclonal-based 
C)  Heterophile antibody-based 
D)  Alloantibody-based 
A)  Polyclonal-based 
B)  Monoclonal-based 
C)  Heterophile antibody-based 
D)  Alloantibody-based

Question 11

Select the test that uses IgG-sensitized red cells (check cells).

Accepted Answer

A)  Antiglobulin test 
B)  D-antigen typing 
C)  Rh-antigen typing 
D)  B-antigen detection 
A)  Antiglobulin test 
B)  D-antigen typing 
C)  Rh-antigen typing 
D)  B-antigen detection

Question 12

Monoclonal antibodies are prepared in:

Accepted Answer

A)  vitro. 
B)  vivo. 
C)  laboratory animals. 
D)  humans. 
A)  vitro. 
B)  vivo. 
C)  laboratory animals. 
D)  humans.

Question 13

How would you interpret the results if both the anti-D reagent and the Rh control were 2+ agglutination reactions?

Accepted Answer

A)  D-positive 
B)  D-negative 
C)  Unable to determine without further testing 
D)  Depends on whether the sample was from a patient or a blood donor 
A)  D-positive 
B)  D-negative 
C)  Unable to determine without further testing 
D)  Depends on whether the sample was from a patient or a blood donor

Question 14

Which of the following red cell antigens do proteolytic enzymes destroy?

Accepted Answer

A)  Rh system antigens 
B)  Antigens Fyᵃ and Fyᵇ in the Duffy system 
C)  Antigens in the Kidd system 
D)  Lewis system antigens 
A)  Rh system antigens 
B)  Antigens Fyᵃ and Fyᵇ in the Duffy system 
C)  Antigens in the Kidd system 
D)  Lewis system antigens

Question 15

Which of the following items provides evidence for reagent red cell deterioration?

Accepted Answer

A)  Spontaneous agglutination 
B)  Significant hemolysis 
C)  Loss of agglutination strength over time 
D)  All of the above 
A)  Spontaneous agglutination 
B)  Significant hemolysis 
C)  Loss of agglutination strength over time 
D)  All of the above

Question 16

Where are product limitations and technical considerations for each reagent located?

Accepted Answer

A)  Standard operating procedure 
B)  Product insert 
C)  Food and Drug Administration code of regulations 
D)  AABB standards 
A)  Standard operating procedure 
B)  Product insert 
C)  Food and Drug Administration code of regulations 
D)  AABB standards

Question 17

In the solid-phase red cell adherence test,how does a negative test appear?

Accepted Answer

A)  A button of cells on the bottom of the well 
B)  Adherence of cells along the sides and bottom of the wells 
C)  Hemolysis of red cells 
D)  A line of cells along the top of the well 
A)  A button of cells on the bottom of the well 
B)  Adherence of cells along the sides and bottom of the wells 
C)  Hemolysis of red cells 
D)  A line of cells along the top of the well

Question 18

What temperature is used for incubation in the indirect antihuman globulin test?

Accepted Answer

A)  22° C 
B)  37° C 
C)  4° C 
D)  56° C 
A)  22° C 
B)  37° C 
C)  4° C 
D)  56° C

Question 19

Solid-phase red cell adherence used for antibody detection has an advantage over tube testing because:

Accepted Answer

A)  there is no washing involved. 
B)  incubation time is not necessary. 
C)  the endpoint is more clearly defined. 
D)  indicator cells (IgG-coated cells) are not necessary. 
A)  there is no washing involved. 
B)  incubation time is not necessary. 
C)  the endpoint is more clearly defined. 
D)  indicator cells (IgG-coated cells) are not necessary.

Question 20

What immunoglobulin class reacts best by antiglobulin testing?

Accepted Answer

A)  IgM 
B)  IgA 
C)  IgE 
D)  IgG 
A)  IgM 
B)  IgA 
C)  IgE 
D)  IgG

What is the purpose of adding antibody-sensitized red cells following the antiglobulin test?

MATCHING Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent used in the identification of ABO antibodies

Rouleaux is a false-positive reaction caused by elevated serum protein levels.Which of the following tests would not likely be affected by an elevated protein level?

Which of the following statements is true regarding IgG-sensitized red cells?

Why is polyethylene glycol reagent added to the screen or panel?

Select the antiglobulin test that best fits the descriptions below.A selection may be used more than once. -Tests for certain clinical conditions such as hemolytic disease of the newborn and autoimmune hemolytic anemia

Select the antiglobulin test that best fits the descriptions below.A selection may be used more than once. -Incubation step is not necessary

Following centrifugation of the gel card,red cells are evenly dispersed throughout one of the microtubes.This reaction could be graded as a:

What specificities does polyspecific antihuman globulin contain?

What reagent contains antibodies to multiple antigenic epitopes?

Select the test that uses IgG-sensitized red cells (check cells).

Monoclonal antibodies are prepared in:

How would you interpret the results if both the anti-D reagent and the Rh control were 2+ agglutination reactions?

Which of the following red cell antigens do proteolytic enzymes destroy?

Which of the following items provides evidence for reagent red cell deterioration?

Where are product limitations and technical considerations for each reagent located?

In the solid-phase red cell adherence test,how does a negative test appear?

What temperature is used for incubation in the indirect antihuman globulin test?

Solid-phase red cell adherence used for antibody detection has an advantage over tube testing because:

What immunoglobulin class reacts best by antiglobulin testing?

Quality Assurance and Regulation of the Blood Industry and Safety Issues in the Blood Bank

Immunology: Basic Principles and Applications in the Blood Bank

Genetic Principles in Blood Banking

ABO and H Blood Group Systems and Secretor Status

Rh Blood Group System

Other Red Cell Blood Group Systems, Human Leukocyte Antigens, and Platelet Antigens

Antibody Detection and Identification

Compatibility Testing

Blood Bank Automation for Transfusion Services

Adverse Complications of Transfusions

Hemolytic Disease of the Fetus and Newborn

Donor Selection and Phlebotomy

Testing of Donor Blood

Blood Component Preparation and Therapy

Transfusion Therapy in Selected Patients

Filters

Exam 3: Blood Banking Reagents: Overview and Applications

What is the purpose of adding antibody-sensitized red cells following the antiglobulin test?

MATCHING Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent used in the identification of ABO antibodies

Rouleaux is a false-positive reaction caused by elevated serum protein levels.Which of the following tests would not likely be affected by an elevated protein level?

Which of the following statements is true regarding IgG-sensitized red cells?

Why is polyethylene glycol reagent added to the screen or panel?

Select the antiglobulin test that best fits the descriptions below.A selection may be used more than once. -Tests for certain clinical conditions such as hemolytic disease of the newborn and autoimmune hemolytic anemia

Select the antiglobulin test that best fits the descriptions below.A selection may be used more than once. -Incubation step is not necessary

Following centrifugation of the gel card,red cells are evenly dispersed throughout one of the microtubes.This reaction could be graded as a:

What specificities does polyspecific antihuman globulin contain?

What reagent contains antibodies to multiple antigenic epitopes?

Select the test that uses IgG-sensitized red cells (check cells).

Monoclonal antibodies are prepared in:

How would you interpret the results if both the anti-D reagent and the Rh control were 2+ agglutination reactions?

Which of the following red cell antigens do proteolytic enzymes destroy?

Which of the following items provides evidence for reagent red cell deterioration?

Where are product limitations and technical considerations for each reagent located?

In the solid-phase red cell adherence test,how does a negative test appear?

What temperature is used for incubation in the indirect antihuman globulin test?

Solid-phase red cell adherence used for antibody detection has an advantage over tube testing because:

What immunoglobulin class reacts best by antiglobulin testing?

Quality Assurance and Regulation of the Blood Industry and Safety Issues in the Blood Bank

Immunology: Basic Principles and Applications in the Blood Bank

Genetic Principles in Blood Banking

ABO and H Blood Group Systems and Secretor Status

Rh Blood Group System

Other Red Cell Blood Group Systems, Human Leukocyte Antigens, and Platelet Antigens

Antibody Detection and Identification

Compatibility Testing

Blood Bank Automation for Transfusion Services

Adverse Complications of Transfusions

Hemolytic Disease of the Fetus and Newborn

Donor Selection and Phlebotomy

Testing of Donor Blood

Blood Component Preparation and Therapy

Transfusion Therapy in Selected Patients

Filters