Question 1

Chang was describing a laboratory instrument that utilizes fluorescent dyes, DNA fragments, electrical field, etc. What is the name of this device?

Accepted Answer

A)  Thermocouple 
B)  DNA Sequencer 
C)  Spectrophotometer 
D)  Micropipettor 
E)  Sonicator 
A)  Thermocouple 
B)  DNA Sequencer 
C)  Spectrophotometer 
D)  Micropipettor 
E)  Sonicator

Question 2

DNA is classified as an acid and is negatively charged because….

Accepted Answer

A)  The phosphate group grabs onto hydrogen atoms 
B)  The deoxyribose group donates hydrogen atoms from the hydroxyl group 
C)  The phosphate group donates hydrogen atoms from the hydroxyl group 
D)  The deoxyribose group loses an oxygen atom at the 2' carbon 
E)  None of the above 
A)  The phosphate group grabs onto hydrogen atoms 
B)  The deoxyribose group donates hydrogen atoms from the hydroxyl group 
C)  The phosphate group donates hydrogen atoms from the hydroxyl group 
D)  The deoxyribose group loses an oxygen atom at the 2' carbon 
E)  None of the above

Question 3

Scientists at Cal Tech (Pasadena, Ca. and Applied Biosystems (Foster City, Ca.) improved upon the Sanger sequencing method. Which one of the following statements is not true.

Accepted Answer

A)  Replacing the use of radioactive isotopes with fluorescent dyes 
B)  Detection of these dyes using UV-vis spectrophotometer 
C)  A machine developed by Applied Biosystems that was an automated sequencing instrument 
D)  Employed the Sanger approach with ddNTPs as terminating bases 
E)  Primers were labeled with fluorescent dyes (dye primer approach) 
A)  Replacing the use of radioactive isotopes with fluorescent dyes 
B)  Detection of these dyes using UV-vis spectrophotometer 
C)  A machine developed by Applied Biosystems that was an automated sequencing instrument 
D)  Employed the Sanger approach with ddNTPs as terminating bases 
E)  Primers were labeled with fluorescent dyes (dye primer approach)

Question 4

Compare and contrast a DNA profile vs. an mtDNA sequencing profile.

Accepted Answer

compare-both use a thermocycler, gel ele

Question 5

What did Helen hand over to Jenkins that pertained to the bone recovered by the fisherman?

Accepted Answer

A)  DNA profile 
B)  Information about Rita Graystone 
C)  Confirmation that the skeletal remains was consistent with Rita Graystone 
D)  mitochondrial DNA sequence 
E)  all of the above 
A)  DNA profile 
B)  Information about Rita Graystone 
C)  Confirmation that the skeletal remains was consistent with Rita Graystone 
D)  mitochondrial DNA sequence 
E)  all of the above

Question 6

If a growing chain of DNA is terminated by a ddATP, the complimentary strand is…

Accepted Answer

A)  Adenine 
B)  Thymine 
C)  Guanine 
D)  Cytosine 
E)  Uracil 
A)  Adenine 
B)  Thymine 
C)  Guanine 
D)  Cytosine 
E)  Uracil

Question 7

During a gel electrophoresis run, sequenced fragments migrate …… proportional to size and are detected by a……….

Accepted Answer

A)  Directly, ambient light 
B)  Inversely, sodium light 
C)  Directly, UV light 
D)  Inversely, laser light 
E)  Inversely, spectral light 
A)  Directly, ambient light 
B)  Inversely, sodium light 
C)  Directly, UV light 
D)  Inversely, laser light 
E)  Inversely, spectral light

Question 8

The Sanger method of sequencing known as the chain termination method had several drawbacks. The drawbacks were…..

Accepted Answer

A)  Had to work with radioactive isotopes to tag the terminated fragments 
B)  Time consuming 
C)  Disposal issue involving radioactive materials 
D)  None of the above 
E)  Choice 'a', 'b', 'c' are correct 
A)  Had to work with radioactive isotopes to tag the terminated fragments 
B)  Time consuming 
C)  Disposal issue involving radioactive materials 
D)  None of the above 
E)  Choice 'a', 'b', 'c' are correct

Question 9

The strands of DNA that are being replicated is referred to as the..

Accepted Answer

A)  Template 
B)  Primer 
C)  Nucleotides 
D)  Ribose sugar 
E)  Base pairs 
A)  Template 
B)  Primer 
C)  Nucleotides 
D)  Ribose sugar 
E)  Base pairs

Question 10

A nucleotide is made up of a….

Accepted Answer

A)  alcohol, phosphate, base 
B)  Sugar, hydroxide, base 
C)  Ribose, phosphate, water molecule 
D)  Deoxyribose sugar, phosphate, base 
E)  All of the above 
A)  alcohol, phosphate, base 
B)  Sugar, hydroxide, base 
C)  Ribose, phosphate, water molecule 
D)  Deoxyribose sugar, phosphate, base 
E)  All of the above

Question 11

Explain the similarities and differences between dye primer sequencing vs. dye terminator sequencing.

Accepted Answer

Similarities: both involve fluorescent d

Question 12

As the conversation ended Sanders suggested to Chang

Accepted Answer

A)  that she be reassigned to work with him 
B)  that she continue working with Jenkins 
C)  that she kept the conversation between the two of them private 
D)  all of the above 
E)  choice 'a' & 'c' are correct 
A)  that she be reassigned to work with him 
B)  that she continue working with Jenkins 
C)  that she kept the conversation between the two of them private 
D)  all of the above 
E)  choice 'a' & 'c' are correct

Question 13

……are used in DNA sequence comparison to determine a possible match between evidence DNA to a reference DNA profile.

Accepted Answer

A)  Base pairs 
B)  Ribose sugars 
C)  Phosphate molecules 
D)  Hydrogen bonds between the base pairs 
E)  None of the above 
A)  Base pairs 
B)  Ribose sugars 
C)  Phosphate molecules 
D)  Hydrogen bonds between the base pairs 
E)  None of the above

Question 14

Many of the 9/11 victims were identified by mtDNA sequencing. Considering the conditions of that infamous event what are the advantages and disadvantages of using this form of human identification.

Accepted Answer

advantages-more abundant in the cell, do

Question 15

Nucleotides will be added in a ………..at the…….carbon position of the sugar molecule.

Accepted Answer

A)  Complimentary manner, 5' 
B)  Complimentary manner, 1' 
C)  Complimentary manner, 2' 
D)  Complimentary manner, 10' 
E)  Complimentary manner, 3' 
A)  Complimentary manner, 5' 
B)  Complimentary manner, 1' 
C)  Complimentary manner, 2' 
D)  Complimentary manner, 10' 
E)  Complimentary manner, 3'

Question 16

A deoxyribose sugar that has lost both of its oxygen at the 2' & 3' positions is called a….

Accepted Answer

A)  Nucleoside 
B)  Nucleotide 
C)  Dideoxynucleotide 
D)  Deoxynucleotide 
E)  dNTP 
A)  Nucleoside 
B)  Nucleotide 
C)  Dideoxynucleotide 
D)  Deoxynucleotide 
E)  dNTP

Question 17

Which of the following pieces of physical evidence is the most informative in solving this case?

Accepted Answer

A)  Shoe print casting 
B)  Trace evidence 
C)  Blood spatter patterns 
D)  DNA sequencing 
E)  All of the above 
A)  Shoe print casting 
B)  Trace evidence 
C)  Blood spatter patterns 
D)  DNA sequencing 
E)  All of the above

Chang was describing a laboratory instrument that utilizes fluorescent dyes, DNA fragments, electrical field, etc. What is the name of this device?

DNA is classified as an acid and is negatively charged because….

Scientists at Cal Tech (Pasadena, Ca. and Applied Biosystems (Foster City, Ca.) improved upon the Sanger sequencing method. Which one of the following statements is not true.

Compare and contrast a DNA profile vs. an mtDNA sequencing profile.

What did Helen hand over to Jenkins that pertained to the bone recovered by the fisherman?

If a growing chain of DNA is terminated by a ddATP, the complimentary strand is…

During a gel electrophoresis run, sequenced fragments migrate …… proportional to size and are detected by a……….

The Sanger method of sequencing known as the chain termination method had several drawbacks. The drawbacks were…..

The strands of DNA that are being replicated is referred to as the..

A nucleotide is made up of a….

Explain the similarities and differences between dye primer sequencing vs. dye terminator sequencing.

As the conversation ended Sanders suggested to Chang

……are used in DNA sequence comparison to determine a possible match between evidence DNA to a reference DNA profile.

Many of the 9/11 victims were identified by mtDNA sequencing. Considering the conditions of that infamous event what are the advantages and disadvantages of using this form of human identification.

Nucleotides will be added in a ………..at the…….carbon position of the sugar molecule.

A deoxyribose sugar that has lost both of its oxygen at the 2' & 3' positions is called a….

Which of the following pieces of physical evidence is the most informative in solving this case?

Crime Scene

Lifting Prints

Trace

All About Blood

Dna Quantitation

The Expert Witness

Lies

Ballistics

Histology

Forensic Anthropology

STRS

Population Statistics

Fingerprint Analysis

In the Courtroom

Filters

Exam 11: MTDNA Sequencing

Chang was describing a laboratory instrument that utilizes fluorescent dyes, DNA fragments, electrical field, etc. What is the name of this device?

DNA is classified as an acid and is negatively charged because….

Scientists at Cal Tech (Pasadena, Ca. and Applied Biosystems (Foster City, Ca.) improved upon the Sanger sequencing method. Which one of the following statements is not true.

Compare and contrast a DNA profile vs. an mtDNA sequencing profile.

What did Helen hand over to Jenkins that pertained to the bone recovered by the fisherman?

If a growing chain of DNA is terminated by a ddATP, the complimentary strand is…

During a gel electrophoresis run, sequenced fragments migrate …… proportional to size and are detected by a……….

The Sanger method of sequencing known as the chain termination method had several drawbacks. The drawbacks were…..

The strands of DNA that are being replicated is referred to as the..

A nucleotide is made up of a….

Explain the similarities and differences between dye primer sequencing vs. dye terminator sequencing.

As the conversation ended Sanders suggested to Chang

……are used in DNA sequence comparison to determine a possible match between evidence DNA to a reference DNA profile.

Many of the 9/11 victims were identified by mtDNA sequencing. Considering the conditions of that infamous event what are the advantages and disadvantages of using this form of human identification.

Nucleotides will be added in a ………..at the…….carbon position of the sugar molecule.

A deoxyribose sugar that has lost both of its oxygen at the 2' & 3' positions is called a….

Which of the following pieces of physical evidence is the most informative in solving this case?

Crime Scene

Lifting Prints

Trace

All About Blood

Dna Quantitation

The Expert Witness

Lies

Ballistics

Histology

Forensic Anthropology

STRS

Population Statistics

Fingerprint Analysis

In the Courtroom

Filters