Question 1

Which of the following choices is an excellent dehydrating agent?

Accepted Answer

A) Dioxane 
B) Triethyl Phosphate 
C) Ethyl alcohol 
D) Acetone 
A) Dioxane 
B) Triethyl Phosphate 
C) Ethyl alcohol 
D) Acetone A

Question 2

The paraffin oven is maintained at what temperature?

Accepted Answer

A) 5-10 degrees Celsius above the melting point of the paraffin used 
B) 10-12 degrees Celsius above the melting point of the paraffin used 
C) 2-5 degrees Celsius above the melting point of the paraffin used 
D) 4-8 degrees Celsius above the melting point of the paraffin used 
A) 5-10 degrees Celsius above the melting point of the paraffin used 
B) 10-12 degrees Celsius above the melting point of the paraffin used 
C) 2-5 degrees Celsius above the melting point of the paraffin used 
D) 4-8 degrees Celsius above the melting point of the paraffin used C

Question 3

Calcium and other calcified materials may be removed from the tissue by any of the following agents, except:

Accepted Answer

A) Acids 
B) Electrophoresis 
C) Chelating agents 
D) Oils 
A) Acids 
B) Electrophoresis 
C) Chelating agents 
D) Oils D

Question 4

Which of the following agent is recommended for central nervous system tissues and other cytological studies?

Accepted Answer

A) Chloroform 
B) Benzene 
C) Cedarwood oil 
D) Clove oil 
A) Chloroform 
B) Benzene 
C) Cedarwood oil 
D) Clove oil

Question 5

This is a purified form of nitrocellulose soluble in many solvents.

Accepted Answer

A) Paraffin wax 
B) Celloidin wax 
C) Gelatin 
D) Paraplast 
A) Paraffin wax 
B) Celloidin wax 
C) Gelatin 
D) Paraplast

Question 6

What process is preferred for processing whole eye sections?

Accepted Answer

A) Wet Celloidin method 
B) Dry Celloidin method 
C) Nitrocellulose method 
D) Gelatin 
A) Wet Celloidin method 
B) Dry Celloidin method 
C) Nitrocellulose method 
D) Gelatin

Question 7

All of the following statements are true about the ideal characteristics of a clearing agent, except:

Accepted Answer

A) It should make tissues transparent. 
B) It should not dissolve out Aniline dyes 
C) It should not evaporate quickly in a water bath. 
D) It should be miscible with water to promote the rapid removal of the dehydrating agent. 
A) It should make tissues transparent. 
B) It should not dissolve out Aniline dyes 
C) It should not evaporate quickly in a water bath. 
D) It should be miscible with water to promote the rapid removal of the dehydrating agent.

Question 8

This type of embedding mold is normally utilized for embedding celloidin blocks, but is equally useful for paraffin wax blocks.

Accepted Answer

A) Plastic ice trays 
B) Paper boats 
C) Compound embedding unit 
D) Leuckhart's embedding mold 
A) Plastic ice trays 
B) Paper boats 
C) Compound embedding unit 
D) Leuckhart's embedding mold

Question 9

All of the following statements about ion exchange resins are correct, except:

Accepted Answer

A) The degree of decalcification can be measured by chemical means. 
B) Cellular detail is well-preserved. 
C) Decalcification is hastened. 
D) Daily washing of solutions is eliminated. 
A) The degree of decalcification can be measured by chemical means. 
B) Cellular detail is well-preserved. 
C) Decalcification is hastened. 
D) Daily washing of solutions is eliminated.

Question 10

This type of clearing agent rapidly clears tissues but it is extremely toxic to man and may be carcinogenic. What clearing agent is this?

Accepted Answer

A) Toluene 
B) Benzene 
C) Xylene 
D) Chloroform 
A) Toluene 
B) Benzene 
C) Xylene 
D) Chloroform

Question 11

All of the following statements are true about clearing agents, except:

Accepted Answer

A) Removal of the fixative from tissue. 
B) Removal of alcohol from the tissue. 
C) Improve refractive index. 
D) Both A and C 
A) Removal of the fixative from tissue. 
B) Removal of alcohol from the tissue. 
C) Improve refractive index. 
D) Both A and C

Question 12

This is the process by which the infiltrate tissue is place into a precisely arranged position in a mold containing a medium which is then allowed to solidify.

Accepted Answer

A) Impregnation 
B) Casting 
C) Embedding 
D) Both B and C 
A) Impregnation 
B) Casting 
C) Embedding 
D) Both B and C

Question 13

All of the following are disposable embedding molds, except:

Accepted Answer

A) Peel away 
B) Plastic embedding rings and base molds 
C) Plastic ice trays 
D) Paper boats 
A) Peel away 
B) Plastic embedding rings and base molds 
C) Plastic ice trays 
D) Paper boats

Question 14

This is a disposable, thin plastic embedding mold that is available in 3 different sizes.

Accepted Answer

A) Plastic ice trays 
B) Paper boats 
C) Peel away 
D) None of the above 
A) Plastic ice trays 
B) Paper boats 
C) Peel away 
D) None of the above

Question 15

This is the procedure whereby lime salts are removed from the tissues following after fixation.

Accepted Answer

A) Dehydration 
B) Decalcification 
C) Impregnation 
D) Clearing 
A) Dehydration 
B) Decalcification 
C) Impregnation 
D) Clearing

Question 16

This is the process whereby the clearing agent is completely removed from the tissue and is replaced by a medium that will completely fill all the tissue cavities.

Accepted Answer

A) Embedding 
B) Impregnation 
C) Mounting 
D) Sectioning 
A) Embedding 
B) Impregnation 
C) Mounting 
D) Sectioning

Question 17

What clearing agent is commonly used for both embedding and mounting procedures?

Accepted Answer

A) Xylene 
B) Carbon tetrachloride 
C) Clove oil 
D) Aniline oil 
A) Xylene 
B) Carbon tetrachloride 
C) Clove oil 
D) Aniline oil

Question 18

What clearing agent should be used when the tissue is to be cleared directly from water, as in a frozen section?

Accepted Answer

A) Xylene 
B) Cedarwood 
C) Glycerine 
D) Dioxane 
A) Xylene 
B) Cedarwood 
C) Glycerine 
D) Dioxane

Question 19

Which of the following best describes the process used in decalcification whereby positively charged calcium ions are attracted to a negative electrode and subsequently removed from the decalcifying solution?

Accepted Answer

A) Acids 
B) Ion exchange 
C) Chelation 
D) Electrophoresis 
A) Acids 
B) Ion exchange 
C) Chelation 
D) Electrophoresis

Question 20

What is the purpose of 1% phenol in Gelatin impregnation?

Accepted Answer

A) To completely wash out the fixative 
B) To harden the tissue 
C) To prevent the growth of molds 
D) To solidify the gelatin 
A) To completely wash out the fixative 
B) To harden the tissue 
C) To prevent the growth of molds 
D) To solidify the gelatin

Which of the following choices is an excellent dehydrating agent?

The paraffin oven is maintained at what temperature?

Calcium and other calcified materials may be removed from the tissue by any of the following agents, except:

Which of the following agent is recommended for central nervous system tissues and other cytological studies?

This is a purified form of nitrocellulose soluble in many solvents.

What process is preferred for processing whole eye sections?

All of the following statements are true about the ideal characteristics of a clearing agent, except:

This type of embedding mold is normally utilized for embedding celloidin blocks, but is equally useful for paraffin wax blocks.

All of the following statements about ion exchange resins are correct, except:

This type of clearing agent rapidly clears tissues but it is extremely toxic to man and may be carcinogenic. What clearing agent is this?

All of the following statements are true about clearing agents, except:

This is the process by which the infiltrate tissue is place into a precisely arranged position in a mold containing a medium which is then allowed to solidify.

All of the following are disposable embedding molds, except:

This is a disposable, thin plastic embedding mold that is available in 3 different sizes.

This is the procedure whereby lime salts are removed from the tissues following after fixation.

This is the process whereby the clearing agent is completely removed from the tissue and is replaced by a medium that will completely fill all the tissue cavities.

What clearing agent is commonly used for both embedding and mounting procedures?

What clearing agent should be used when the tissue is to be cleared directly from water, as in a frozen section?

Which of the following best describes the process used in decalcification whereby positively charged calcium ions are attracted to a negative electrode and subsequently removed from the decalcifying solution?

What is the purpose of 1% phenol in Gelatin impregnation?

Fixation

Laboratory Operations

Microtomy

Staining

Filters

Exam 4: Processing and Embedding

Which of the following choices is an excellent dehydrating agent?

The paraffin oven is maintained at what temperature?

Calcium and other calcified materials may be removed from the tissue by any of the following agents, except:

Which of the following agent is recommended for central nervous system tissues and other cytological studies?

This is a purified form of nitrocellulose soluble in many solvents.

What process is preferred for processing whole eye sections?

All of the following statements are true about the ideal characteristics of a clearing agent, except:

This type of embedding mold is normally utilized for embedding celloidin blocks, but is equally useful for paraffin wax blocks.

All of the following statements about ion exchange resins are correct, except:

This type of clearing agent rapidly clears tissues but it is extremely toxic to man and may be carcinogenic. What clearing agent is this?

All of the following statements are true about clearing agents, except:

This is the process by which the infiltrate tissue is place into a precisely arranged position in a mold containing a medium which is then allowed to solidify.

All of the following are disposable embedding molds, except:

This is a disposable, thin plastic embedding mold that is available in 3 different sizes.

This is the procedure whereby lime salts are removed from the tissues following after fixation.

This is the process whereby the clearing agent is completely removed from the tissue and is replaced by a medium that will completely fill all the tissue cavities.

What clearing agent is commonly used for both embedding and mounting procedures?

What clearing agent should be used when the tissue is to be cleared directly from water, as in a frozen section?

Which of the following best describes the process used in decalcification whereby positively charged calcium ions are attracted to a negative electrode and subsequently removed from the decalcifying solution?

What is the purpose of 1% phenol in Gelatin impregnation?

Fixation

Laboratory Operations

Microtomy

Staining

Filters