Question 1

Refer to the figure above and then answer the questions that follow.
-Which of the following is a very important step in PCR? Refer to the figure above for help.

Accepted Answer

A)  DNA polymerase makes complementary strands of DNA. 
B)  Primers separate the double strands of DNA. 
C)  A single replication cycle is performed to amplify DNA. 
D)  DNA strands are bonded together by heat. 
A)  DNA polymerase makes complementary strands of DNA. 
B)  Primers separate the double strands of DNA. 
C)  A single replication cycle is performed to amplify DNA. 
D)  DNA strands are bonded together by heat.

Question 2

A hollow fluid-filled ball of embryonic cells is called

Accepted Answer

A)  a sperm cell 
B)  an egg cell 
C)  a blastocyst 
D)  a stem cell 
A)  a sperm cell 
B)  an egg cell 
C)  a blastocyst 
D)  a stem cell

Question 3

Refer to the figure above and then answer the question that follows.
-Which statement about restriction enzymes is true?

Accepted Answer

A)  Millions of different ones have been identified so far. 
B)  They cut only one strand of DNA to produce a fragment. 
C)  They are isolated from human cells, which naturally produce them. 
D)  They cut any DNA with their recognition sequence. 
A)  Millions of different ones have been identified so far. 
B)  They cut only one strand of DNA to produce a fragment. 
C)  They are isolated from human cells, which naturally produce them. 
D)  They cut any DNA with their recognition sequence.

Question 4

Write an explanation for why the DNA of a bacteriophage, a virus that infects bacterial cells, would be a good cloning vector. (Think about the qualities of a virus, and also the qualities of a plasmid).

Accepted Answer

As a virus, a bacteriophage has a piece

Question 5

Which of the following techniques is usually needed in the use of genetic evidence in court cases?

Accepted Answer

A)  cloning 
B)  PCR 
C)  restriction enzymes 
D)  plasmids 
A)  cloning 
B)  PCR 
C)  restriction enzymes 
D)  plasmids

Question 6

Someone with a billion dollars and an ego to match would be able to have himself cloned now or in the near future.

Accepted Answer

A) True 
 B)False

Question 7

Refer to the figure above and then answer the questions that follow.
-DNA sequencing is most commonly used to determine:

Accepted Answer

A)  the sites of DNA fragments cut with restriction enzymes. 
B)  the number of VNTR repeats in a chromosome section. 
C)  the sequence recognized by a restriction enzyme. 
D)  the sequence of bases in a DNA segment. 
A)  the sites of DNA fragments cut with restriction enzymes. 
B)  the number of VNTR repeats in a chromosome section. 
C)  the sequence recognized by a restriction enzyme. 
D)  the sequence of bases in a DNA segment.

Question 8

Match each technique with one of the steps in its process.
-PCR

Accepted Answer

A)  Use restriction enzymes to cut plasmid DNA and insert human gene. 
B)  Samples of DNA are compared with respect to short tandem repeats. 
C)  DNA is heated to separate strands. 
D)  A human gene is inserted into a viral vector. 
E)  All nuclear DNA is removed from an egg. 
A)  Use restriction enzymes to cut plasmid DNA and insert human gene. 
B)  Samples of DNA are compared with respect to short tandem repeats. 
C)  DNA is heated to separate strands. 
D)  A human gene is inserted into a viral vector. 
E)  All nuclear DNA is removed from an egg.

Question 9

Some people are violently opposed to the production and use of genetically modified (GM) foods because:

Accepted Answer

A)  numerous allergic reactions to these foods have been demonstrated. 
B)  the foods increase productivity and thus fewer people will starve. 
C)  the American Medical Association requires labeling of all GM foods. 
D)  their genes may spread to nearby wild organisms. 
A)  numerous allergic reactions to these foods have been demonstrated. 
B)  the foods increase productivity and thus fewer people will starve. 
C)  the American Medical Association requires labeling of all GM foods. 
D)  their genes may spread to nearby wild organisms.

Question 10

Which of the following is not a valid argument against the increased use of biotechnology information and procedures?

Accepted Answer

A)  People will worry because they know they have a disease for which there is no treatment. 
B)  Life insurance companies might use genetic predispositions to refuse coverage to some people. 
C)  The money invested in this technology could be put to better uses. 
D)  It is a new technology that has not been sufficiently tested. 
A)  People will worry because they know they have a disease for which there is no treatment. 
B)  Life insurance companies might use genetic predispositions to refuse coverage to some people. 
C)  The money invested in this technology could be put to better uses. 
D)  It is a new technology that has not been sufficiently tested.

Question 11

Match each technique with one of the steps in its process.
-Gene therapy

Accepted Answer

A)  Use restriction enzymes to cut plasmid DNA and insert human gene. 
B)  Samples of DNA are compared with respect to short tandem repeats. 
C)  DNA is heated to separate strands. 
D)  A human gene is inserted into a viral vector. 
E)  All nuclear DNA is removed from an egg. 
A)  Use restriction enzymes to cut plasmid DNA and insert human gene. 
B)  Samples of DNA are compared with respect to short tandem repeats. 
C)  DNA is heated to separate strands. 
D)  A human gene is inserted into a viral vector. 
E)  All nuclear DNA is removed from an egg.

Question 12

Therapeutic cloning is used:

Accepted Answer

A)  to produce transgenic bacteria. 
B)  human embryonic stem cells to treat disease and injury. 
C)  large amounts of transgenic DNA. 
D)  insulin. 
A)  to produce transgenic bacteria. 
B)  human embryonic stem cells to treat disease and injury. 
C)  large amounts of transgenic DNA. 
D)  insulin.

Question 13

Biotechnology can be defined as the use of living organisms to create products or to facilitate process. Restriction enzymes are one kind of tool required by this technology. What roles do restriction enzymes play?

Accepted Answer

A)  They break down all DNA molecules randomly. 
B)  They are used to break down the cell membrane of bacterial cell. 
C)  They break down all protein molecules inside the bacterial cell. 
D)  They cut DNA at specific sites. 
A)  They break down all DNA molecules randomly. 
B)  They are used to break down the cell membrane of bacterial cell. 
C)  They break down all protein molecules inside the bacterial cell. 
D)  They cut DNA at specific sites.

Question 14

Which choice is an example of two "clones"?

Accepted Answer

A)  a bacterial plasmid and a genomic DNA fragment, both cut with the same enzyme 
B)  a human gene on a chromosome, and an exact copy in a recombinant plasmid 
C)  a bacterial cell with a plasmid, and a bacterial cell without a plasmid 
D)  a sheep, and an egg from that sheep that was used in reproductive cloning 
A)  a bacterial plasmid and a genomic DNA fragment, both cut with the same enzyme 
B)  a human gene on a chromosome, and an exact copy in a recombinant plasmid 
C)  a bacterial cell with a plasmid, and a bacterial cell without a plasmid 
D)  a sheep, and an egg from that sheep that was used in reproductive cloning

Question 15

What is one of the advantages of producing human growth hormone (HGH) from a cloned gene in a bacterial cell?

Accepted Answer

A)  The product can be administered by infecting a person with the recombinant bacteria. 
B)  A gene cloned from mice, rather than from humans can be used. 
C)  The bacterial protein has a different amino acid sequence that is more effective. 
D)  Larger quantities are available. 
E)  As the protein is introduced therapeutically, it transfers the HGH gene as well. 
A)  The product can be administered by infecting a person with the recombinant bacteria. 
B)  A gene cloned from mice, rather than from humans can be used. 
C)  The bacterial protein has a different amino acid sequence that is more effective. 
D)  Larger quantities are available. 
E)  As the protein is introduced therapeutically, it transfers the HGH gene as well.

Question 16

Match each technique with one of the steps in its process.
-producing recombinant DNA

Accepted Answer

A)  Use restriction enzymes to cut plasmid DNA and insert human gene. 
B)  Samples of DNA are compared with respect to short tandem repeats. 
C)  DNA is heated to separate strands. 
D)  A human gene is inserted into a viral vector. 
E)  All nuclear DNA is removed from an egg. 
A)  Use restriction enzymes to cut plasmid DNA and insert human gene. 
B)  Samples of DNA are compared with respect to short tandem repeats. 
C)  DNA is heated to separate strands. 
D)  A human gene is inserted into a viral vector. 
E)  All nuclear DNA is removed from an egg.

Question 17

In PCR, what is the function of the primers?

Accepted Answer

A)  They extract DNA from cells. 
B)  They introduce DNA into bacteria. 
C)  They indicate where DNA synthesis should start. 
D)  They are the enzymes that replicate DNA. 
A)  They extract DNA from cells. 
B)  They introduce DNA into bacteria. 
C)  They indicate where DNA synthesis should start. 
D)  They are the enzymes that replicate DNA.

Question 18

Refer to the figure above and then answer the questions that follow.
-What is the goal of PCR? Refer to the figure above for help.

Accepted Answer

A)  separation of DNA by size 
B)  making many copies of a transgenic animal 
C)  introduction of DNA into reproductively cloned animals 
D)  copying DNA when the starting sample is small 
A)  separation of DNA by size 
B)  making many copies of a transgenic animal 
C)  introduction of DNA into reproductively cloned animals 
D)  copying DNA when the starting sample is small

Question 19

A primer is used in which of the biotechnology processes?

Accepted Answer

A)  PCR 
B)  cloning 
C)  cleaving DNA 
D)  recombining DNA 
A)  PCR 
B)  cloning 
C)  cleaving DNA 
D)  recombining DNA

Question 20

A "transgenic animal:

Accepted Answer

A)  is the offspring of a clone. 
B)  has been amplified by PCR. 
C)  contains a foreign gene. 
D)  has had all its chromosomes removed. 
A)  is the offspring of a clone. 
B)  has been amplified by PCR. 
C)  contains a foreign gene. 
D)  has had all its chromosomes removed.

Exam 15: Biotechnology

Refer to the figure above and then answer the questions that follow. -Which of the following is a very important step in PCR? Refer to the figure above for help.

A hollow fluid-filled ball of embryonic cells is called

Refer to the figure above and then answer the question that follows. -Which statement about restriction enzymes is true?

Write an explanation for why the DNA of a bacteriophage, a virus that infects bacterial cells, would be a good cloning vector. (Think about the qualities of a virus, and also the qualities of a plasmid).

Which of the following techniques is usually needed in the use of genetic evidence in court cases?

Someone with a billion dollars and an ego to match would be able to have himself cloned now or in the near future.

Refer to the figure above and then answer the questions that follow. -DNA sequencing is most commonly used to determine:

Match each technique with one of the steps in its process. -PCR

Some people are violently opposed to the production and use of genetically modified (GM) foods because:

Which of the following is not a valid argument against the increased use of biotechnology information and procedures?

Match each technique with one of the steps in its process. -Gene therapy

Therapeutic cloning is used:

Biotechnology can be defined as the use of living organisms to create products or to facilitate process. Restriction enzymes are one kind of tool required by this technology. What roles do restriction enzymes play?

Which choice is an example of two "clones"?

What is one of the advantages of producing human growth hormone (HGH) from a cloned gene in a bacterial cell?

Match each technique with one of the steps in its process. -producing recombinant DNA

In PCR, what is the function of the primers?

Refer to the figure above and then answer the questions that follow. -What is the goal of PCR? Refer to the figure above for help.

A primer is used in which of the biotechnology processes?

A "transgenic animal:

A Guide to the Natural World

Chemistry, Water and PH

Lifes Components: Biological Molecules

Lifes Home: The Cell

Lifes Border: The Plasma Membrane

An Introduction to Energy

Deriving Energy From Food

Photosynthesis

Genetics and Cell Division

Preparing for Sexual Reproduction: Meiosis

Mendel and His Discoveries

Chromosomes and Inheritance

Dna Structure and Replication

Transcription Translation and Regulation

Charles Darwin, Evolutionary Thought, and the Evidence for Evolution

Microevolution

Macroevolution

The History of Life on Earth

The Evolution of Human Beings

The Diversity of Life 1

The Diversity of Life 2

The Diversity of Life 3

An Introduction to Flowering Plants

Form and Function in Flowering Plants

The Integumentary Skeletal and Muscular Systems

The Nervous and Endocrine System

The Immune System

Blood and Breath

Digestion, Nutrition and Elimination

Animal Development

Human Reproduction

Populations in Ecology

Communities in Ecology

Ecosystems and Biomes

Animal Behavior

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