Question 1

Cold autoantibodies are typically of which specificity?

Accepted Answer

A) M 
B) N 
C) I 
D) Leb 
A) M 
B) N 
C) I 
D) Leb

Question 2

An anti-Fya was identified in a patient's serum.The patient's red cells phenotyped as Fya positive using commercial antisera.The next step is to:

Accepted Answer

A) repeat the panel to confirm the antibody. 
B) report the antibody because this result is normal. 
C) investigate a recent transfusion history. 
D) wash the cells and use monoclonal anti-Fya antibodies. 
A) repeat the panel to confirm the antibody. 
B) report the antibody because this result is normal. 
C) investigate a recent transfusion history. 
D) wash the cells and use monoclonal anti-Fya antibodies.

Question 3

Antibody screening cells are positive at the antihuman globulin phase of testing.The first step of the investigation should be to:

Accepted Answer

A) check transfusion and pregnancy history. 
B) perform a direct antiglobulin test using anti-C3. 
C) repeat the ABO typing. 
D) crossmatch units until one is compatible. 
A) check transfusion and pregnancy history. 
B) perform a direct antiglobulin test using anti-C3. 
C) repeat the ABO typing. 
D) crossmatch units until one is compatible.

Question 4

An autoadsorption may be performed to investigate underlying autoantibodies.When is this procedure acceptable?

Accepted Answer

A) When the autoantibody is reactive at 4° C 
B) When the patient has not been recently transfused 
C) Only if complement is coating the red cells 
D) When the eluate is negative 
A) When the autoantibody is reactive at 4° C 
B) When the patient has not been recently transfused 
C) Only if complement is coating the red cells 
D) When the eluate is negative

Question 5

If all the panel cells were reactive at the same strength at the antihuman globulin phase,no negative reactions were observed,and the autocontrol was negative,what should be suspected?

Accepted Answer

A) Multiple antibody specificities 
B) Warm autoantibody 
C) Antibody to a low-frequency antigen 
D) Antibody to a high-frequency antigen 
A) Multiple antibody specificities 
B) Warm autoantibody 
C) Antibody to a low-frequency antigen 
D) Antibody to a high-frequency antigen

Question 6

An antibody was detected at immediate spin and 37° C that appeared to have anti-Leb specificity.To confirm the antibody identity and determine if there were other antibodies in the serum,a Lewis neutralization technique was performed.Results of the control are as follows: Reaction with Le(b)positive cells
Patient serum + Lewis substance 0
Patient serum + saline control 1+
What conclusion can be made from these results?

Accepted Answer

A) anti-Leb is confirmed 
B) the antibody was diluted,therefore no conclusion can be made 
C) the antibody was not neutralized,therefore anti-Leb has not been identified 
D) an antibody other than anti-Leb is most likely in the serum 
A) anti-Leb is confirmed 
B) the antibody was diluted,therefore no conclusion can be made 
C) the antibody was not neutralized,therefore anti-Leb has not been identified 
D) an antibody other than anti-Leb is most likely in the serum

Question 7

Anti-D,anti-K,and anti-Jka are the antibodies that are tentatively identified on a panel after initially ruling out on negative cells.What selected cell from another panel should be chosen to confirm the presence of anti-K?

Accepted Answer

A) K-,D+,Jk(a+) 
B) K+,D+,Jk(a+) 
C) K+,D-,Jk(a+) 
D) K+,D-,Jk(a-) 
A) K-,D+,Jk(a+) 
B) K+,D+,Jk(a+) 
C) K+,D-,Jk(a+) 
D) K+,D-,Jk(a-)

Question 8

What is the most important concern when trying to identify antibodies in a patient with a warm autoantibody?

Accepted Answer

A) Identifying the specificity of the autoantibody 
B) Determining whether there are underlying alloantibodies 
C) Identifying the antibody found in the eluate 
D) Determining whether complement is binding to the autologous red cells 
A) Identifying the specificity of the autoantibody 
B) Determining whether there are underlying alloantibodies 
C) Identifying the antibody found in the eluate 
D) Determining whether complement is binding to the autologous red cells

Question 9

Rh system antibodies characteristically give:

Accepted Answer

A) mixed-field reactions on panels. 
B) weak reactions with panel cells. 
C) strong reactions with panel cells when read at immediate spin phase. 
D) reactions that are enhanced with enzymes. 
A) mixed-field reactions on panels. 
B) weak reactions with panel cells. 
C) strong reactions with panel cells when read at immediate spin phase. 
D) reactions that are enhanced with enzymes.

Question 10

Which of the following situations can be found in a classic case of autoimmune hemolytic anemia?

Accepted Answer

A) Positive direct antiglobulin test 
B) False-positive Fya phenotyping 
C) Crossmatch incompatibility at antihuman globulin 
D) All of the above 
A) Positive direct antiglobulin test 
B) False-positive Fya phenotyping 
C) Crossmatch incompatibility at antihuman globulin 
D) All of the above

Cold autoantibodies are typically of which specificity?

An anti-Fy^awas identified in a patient's serum.The patient's red cells phenotyped as Fy^a positive using commercial antisera.The next step is to:

Antibody screening cells are positive at the antihuman globulin phase of testing.The first step of the investigation should be to:

An autoadsorption may be performed to investigate underlying autoantibodies.When is this procedure acceptable?

If all the panel cells were reactive at the same strength at the antihuman globulin phase,no negative reactions were observed,and the autocontrol was negative,what should be suspected?

Anti-D,anti-K,and anti-Jk^a are the antibodies that are tentatively identified on a panel after initially ruling out on negative cells.What selected cell from another panel should be chosen to confirm the presence of anti-K?

What is the most important concern when trying to identify antibodies in a patient with a warm autoantibody?

Rh system antibodies characteristically give:

Which of the following situations can be found in a classic case of autoimmune hemolytic anemia?

Immunology: Basic Principles and Applications in the Blood Bank

Blood Banking Reagents: Overview and Applications

Genetic Principles in Blood Banking

Abo and H Blood Group Systems and Secretor Status

Rh Blood Group System

Other Blood Group Systems

Compatibility Testing

Blood Bank Automation for Transfusion Services

Adverse Complications of Transfusions

Hemolytic Disease of the Fetus and Newborn

Donor Selection and Phlebotomy

Testing of Donor Blood

Blood Component Preparation and Therapy

Transfusion Therapy in Selected Patients

Quality Assurance and Regulation of the Blood Industry and Safety Issues in the Blood Bank

Filters

Exam 7: Antibody Detection and Identification

Cold autoantibodies are typically of which specificity?

An anti-Fya was identified in a patient's serum.The patient's red cells phenotyped as Fya positive using commercial antisera.The next step is to:

Antibody screening cells are positive at the antihuman globulin phase of testing.The first step of the investigation should be to:

An autoadsorption may be performed to investigate underlying autoantibodies.When is this procedure acceptable?

If all the panel cells were reactive at the same strength at the antihuman globulin phase,no negative reactions were observed,and the autocontrol was negative,what should be suspected?

Anti-D,anti-K,and anti-Jka are the antibodies that are tentatively identified on a panel after initially ruling out on negative cells.What selected cell from another panel should be chosen to confirm the presence of anti-K?

What is the most important concern when trying to identify antibodies in a patient with a warm autoantibody?

Rh system antibodies characteristically give:

Which of the following situations can be found in a classic case of autoimmune hemolytic anemia?

Immunology: Basic Principles and Applications in the Blood Bank

Blood Banking Reagents: Overview and Applications

Genetic Principles in Blood Banking

Abo and H Blood Group Systems and Secretor Status

Rh Blood Group System

Other Blood Group Systems

Compatibility Testing

Blood Bank Automation for Transfusion Services

Adverse Complications of Transfusions

Hemolytic Disease of the Fetus and Newborn

Donor Selection and Phlebotomy

Testing of Donor Blood

Blood Component Preparation and Therapy

Transfusion Therapy in Selected Patients

Quality Assurance and Regulation of the Blood Industry and Safety Issues in the Blood Bank

Filters

An anti-Fy^awas identified in a patient's serum.The patient's red cells phenotyped as Fy^a positive using commercial antisera.The next step is to:

Anti-D,anti-K,and anti-Jk^a are the antibodies that are tentatively identified on a panel after initially ruling out on negative cells.What selected cell from another panel should be chosen to confirm the presence of anti-K?