Question 1

FISH allows DNA or RNA to be visualized in its natural location.

Accepted Answer

A) True 
 B)False  True

Question 2

Which statement is not true regarding peptide nucleic acids?

Accepted Answer

A) Peptide nucleic acids are not degraded by nucleases or proteases. 
B) Peptide nucleic acids consist of a polypeptide backbone to which nucleic acid bases are attached as side chains. 
C) Peptide nucleic acids are able to bind to complementary strands of DNA or RNA. 
D) Peptide nucleic acid penetrates cells easily,and therefore has importance in medicine. 
A) Peptide nucleic acids are not degraded by nucleases or proteases. 
B) Peptide nucleic acids consist of a polypeptide backbone to which nucleic acid bases are attached as side chains. 
C) Peptide nucleic acids are able to bind to complementary strands of DNA or RNA. 
D) Peptide nucleic acid penetrates cells easily,and therefore has importance in medicine. D

Question 3

GC base pairs absorb more ultraviolet light than AT base pairs.

Accepted Answer

A) True 
 B)False  True

Question 4

Which of the following fragments of DNA would migrate closest to the wells during agarose gel electrophoresis?

Accepted Answer

A) 1000 bp 
B) 150 bp 
C) 10,000 bp 
D) 650 bp 
A) 1000 bp 
B) 150 bp 
C) 10,000 bp 
D) 650 bp

Question 5

Agarose gel electrophoresis allows the separation of DNA based uponــــــــــــــــــــ .

Accepted Answer

A) charge 
B) base composition 
C) size 
D) pH 
A) charge 
B) base composition 
C) size 
D) pH

Question 6

Which type of blotting uses antibodies to detect proteins on a membrane?

Accepted Answer

A) Southern blot 
B) Northern blot 
C) Western blot 
D) South-Western blot 
A) Southern blot 
B) Northern blot 
C) Western blot 
D) South-Western blot

Question 7

By what technique can individual DNA molecules be visualized?

Accepted Answer

A) electron microscopy and metal shadowing 
B) light microscopy only 
C) agarose gel electrophoresis 
D) Northern blot 
A) electron microscopy and metal shadowing 
B) light microscopy only 
C) agarose gel electrophoresis 
D) Northern blot

Question 8

At which wavelength do the bases of DNA and RNA nucleotides absorb the most light?

Accepted Answer

A) 200 nm 
B) 260 nm 
C) 280 nm 
D) 300 nm 
A) 200 nm 
B) 260 nm 
C) 280 nm 
D) 300 nm

Question 9

Which type of hybridization technique would you use to detect the presence of a specific transcript within a culture of bacterial cells grown under specific growth conditions.

Accepted Answer

A) Eastern blot 
B) Western blot 
C) Northern blot 
D) Southern blot 
A) Eastern blot 
B) Western blot 
C) Northern blot 
D) Southern blot

Question 10

An A260/A280 ratio of less than 1.8 indicatesـــــــــــــــــــــــــ .

Accepted Answer

A) pure DNA 
B) pure RNA 
C) protein contamination of DNA 
D) RNA contamination of DNA 
A) pure DNA 
B) pure RNA 
C) protein contamination of DNA 
D) RNA contamination of DNA

Question 11

What types of labels are added to probes to enable detection during hybridization experiments?

Accepted Answer

A) fluorescence 
B) chemical 
C) radioactive 
D) all of the above 
A) fluorescence 
B) chemical 
C) radioactive 
D) all of the above

FISH allows DNA or RNA to be visualized in its natural location.

Which statement is not true regarding peptide nucleic acids?

GC base pairs absorb more ultraviolet light than AT base pairs.

Which of the following fragments of DNA would migrate closest to the wells during agarose gel electrophoresis?

Agarose gel electrophoresis allows the separation of DNA based uponــــــــــــــــــــ .

Which type of blotting uses antibodies to detect proteins on a membrane?

By what technique can individual DNA molecules be visualized?

At which wavelength do the bases of DNA and RNA nucleotides absorb the most light?

Which type of hybridization technique would you use to detect the presence of a specific transcript within a culture of bacterial cells grown under specific growth conditions.

An A260/A280 ratio of less than 1.8 indicatesـــــــــــــــــــــــــ .

What types of labels are added to probes to enable detection during hybridization experiments?

Cells and Organisms

Basic Genetics

DNA, RNA, and Protein

Genes, genomes, and DNA

Polymerase Chain Reaction

Recombinant DNA Technology

DNA Sequencing

Genomics Systems Biology

Cell Division and DNA Replication

Transcription of Genes

Processing of RNA

Protein Synthesis

Protein Structure and Function

Proteomics

Regulation of Transcription in Prokaryotes

Regulation of Transcription in Eukaryotes

Regulation at the RNA Level

Analysis of Gene Expression

Plasmids

Viruses

Mobile DNA

Mutations and Repair

Recombination

Bacterial Genetics

Molecular Evolution

Filters

Exam 5: Manipulation of Nucleic Acids

FISH allows DNA or RNA to be visualized in its natural location.

Which statement is not true regarding peptide nucleic acids?

GC base pairs absorb more ultraviolet light than AT base pairs.

Which of the following fragments of DNA would migrate closest to the wells during agarose gel electrophoresis?

Agarose gel electrophoresis allows the separation of DNA based uponــــــــــــــــــــ .

Which type of blotting uses antibodies to detect proteins on a membrane?

By what technique can individual DNA molecules be visualized?

At which wavelength do the bases of DNA and RNA nucleotides absorb the most light?

Which type of hybridization technique would you use to detect the presence of a specific transcript within a culture of bacterial cells grown under specific growth conditions.

An A260/A280 ratio of less than 1.8 indicatesـــــــــــــــــــــــــ .

What types of labels are added to probes to enable detection during hybridization experiments?

Cells and Organisms

Basic Genetics

DNA, RNA, and Protein

Genes, genomes, and DNA

Polymerase Chain Reaction

Recombinant DNA Technology

DNA Sequencing

Genomics Systems Biology

Cell Division and DNA Replication

Transcription of Genes

Processing of RNA

Protein Synthesis

Protein Structure and Function

Proteomics

Regulation of Transcription in Prokaryotes

Regulation of Transcription in Eukaryotes

Regulation at the RNA Level

Analysis of Gene Expression

Plasmids

Viruses

Mobile DNA

Mutations and Repair

Recombination

Bacterial Genetics

Molecular Evolution

Filters