Question 1

Explain the meaning and utility of RNA interference.

Accepted Answer

RNA interference (RNAi) is a p

Question 2

A linear strand of DNA is 1,000 bp long. A recognition sequence for the restriction enzyme Eco R1 is located 300 base pairs (bp) from the 5' end of this linear DNA molecule. Digesting this DNA molecule with Eco RI would produce ________.

Accepted Answer

A)  one DNA fragment, 1,000 bp long 
B)  three DNA fragments, two of them 300 bp long and one 400 bp long 
C)  two DNA fragments, one 300 bp long and one 700 bp long 
D)  two fragments, each 500 bp long 
E)  none of the above 
A)  one DNA fragment, 1,000 bp long 
B)  three DNA fragments, two of them 300 bp long and one 400 bp long 
C)  two DNA fragments, one 300 bp long and one 700 bp long 
D)  two fragments, each 500 bp long 
E)  none of the above

Question 3

Which of the following statements about the human genome is INCORRECT?

Accepted Answer

A)  There are approximately 3.1 billion bp in the human genome. 
B)  Human share a majority of genes with other species. 
C)  Less than 2 percent of the genome codes for proteins. 
D)  The human genome contains approximately 100,000 genes. 
E)  The functions of most human genes are still unknown. 
A)  There are approximately 3.1 billion bp in the human genome. 
B)  Human share a majority of genes with other species. 
C)  Less than 2 percent of the genome codes for proteins. 
D)  The human genome contains approximately 100,000 genes. 
E)  The functions of most human genes are still unknown.

Question 4

Which of the following techniques is most efficient for transforming bacterial cells?

Accepted Answer

A)  Gene gun 
B)  ES cell transfer 
C)  Electroporation 
D)  Pronuclear microinjection 
E)  Sperm-mediated transfer 
A)  Gene gun 
B)  ES cell transfer 
C)  Electroporation 
D)  Pronuclear microinjection 
E)  Sperm-mediated transfer

Question 5

Imagine you wanted to use a human genomic DNA library to clone the human gene for insulin. You will be using the rat insulin gene sequence as your DNA probe. In what order would you perform the following steps to accomplish this goal? 1. Use autoradiography to identify colonies containing DNA that hybridized to the probe
2) Grow transformed cells on media with antibiotics and X-gal for blue-white screening
3) Ligate genomic DNA and vector DNA
4) Cut genomic DNA and vector DNA with restriction enzymes
5) Hybridize library DNA with labeled probe for the rat insulin gene
6) Transform bacteria with recombinant plasmid

Accepted Answer

A)  2, 3, 6, 5, 4, 1 
B)  1, 2, 5, 6, 4, 3 
C)  4, 3, 6, 2, 5, 1 
D)  4, 3, 5, 2, 6, 1 
E)  2, 6, 5, 3, 1, 4 
A)  2, 3, 6, 5, 4, 1 
B)  1, 2, 5, 6, 4, 3 
C)  4, 3, 6, 2, 5, 1 
D)  4, 3, 5, 2, 6, 1 
E)  2, 6, 5, 3, 1, 4

Question 6

Which of the following might lead to the generation of several incorrect PCR products?

Accepted Answer

A)  An annealing temperature that is too low 
B)  An annealing temperature that is too high 
C)  Performing 30 cycles of PCR 
D)  Using the correct primers 
E)  Including an extension step 
A)  An annealing temperature that is too low 
B)  An annealing temperature that is too high 
C)  Performing 30 cycles of PCR 
D)  Using the correct primers 
E)  Including an extension step

Question 7

A recombinant DNA molecule is produced artificially and contains sequences ________.

Accepted Answer

A)  from bacteria only 
B)  from yeast only 
C)  from unrelated organisms 
D)  from cells that have been chemically modified 
E)  from prokaryotic genes 
A)  from bacteria only 
B)  from yeast only 
C)  from unrelated organisms 
D)  from cells that have been chemically modified 
E)  from prokaryotic genes

Question 8

Dideoxyribonucleotides (ddNTPs) used for DNA sequencing lack oxygen atoms at ________.

Accepted Answer

A)  the 1' carbon of the pentose sugar 
B)  the 1' and 2' carbons of the pentose sugar 
C)  the 2' and 3' carbons of the pentose sugar 
D)  the 3' and 5' carbons of the pentose sugar 
E)  the 5' carbon of the pentose sugar 
A)  the 1' carbon of the pentose sugar 
B)  the 1' and 2' carbons of the pentose sugar 
C)  the 2' and 3' carbons of the pentose sugar 
D)  the 3' and 5' carbons of the pentose sugar 
E)  the 5' carbon of the pentose sugar

Question 9

Which of the following techniques is the best choice for amplifying DNA?

Accepted Answer

A)  Southern blot analysis 
B)  PCR 
C)  DNA sequencing 
D)  Affinity chromatography 
E)  Microarray analysis 
A)  Southern blot analysis 
B)  PCR 
C)  DNA sequencing 
D)  Affinity chromatography 
E)  Microarray analysis

Question 10

Which of the following techniques is most commonly used to separate and analyze DNA by size?

Accepted Answer

A)  PCR 
B)  Agarose gel electrophoresis 
C)  DNA microarray analysis 
D)  Hybridization 
E)  DNA libraries 
A)  PCR 
B)  Agarose gel electrophoresis 
C)  DNA microarray analysis 
D)  Hybridization 
E)  DNA libraries

Explain the meaning and utility of RNA interference.

A linear strand of DNA is 1,000 bp long. A recognition sequence for the restriction enzyme Eco R1 is located 300 base pairs (bp) from the 5' end of this linear DNA molecule. Digesting this DNA molecule with Eco RI would produce ________.

Which of the following statements about the human genome is INCORRECT?

Which of the following techniques is most efficient for transforming bacterial cells?

Which of the following might lead to the generation of several incorrect PCR products?

A recombinant DNA molecule is produced artificially and contains sequences ________.

Dideoxyribonucleotides (ddNTPs) used for DNA sequencing lack oxygen atoms at ________.

Which of the following techniques is the best choice for amplifying DNA?

Which of the following techniques is most commonly used to separate and analyze DNA by size?

The Biotechnology Century and Its Workforce

An Introduction to Genes and Genomes

Proteins As Products

Microbial Biotechnology

Plant Biotechnology

Animal Biotechnology

Dna Fingerprinting and Forensic Analysis

Bioremediation

Aquatic Biotechnology

Medical Biotechnology

Biotechnology Regulations

Ethics and Biotechnology

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Exam 3: Recombinant Dna Technology and Genomics

Explain the meaning and utility of RNA interference.

A linear strand of DNA is 1,000 bp long. A recognition sequence for the restriction enzyme Eco R1 is located 300 base pairs (bp) from the 5' end of this linear DNA molecule. Digesting this DNA molecule with Eco RI would produce ________.

Which of the following statements about the human genome is INCORRECT?

Which of the following techniques is most efficient for transforming bacterial cells?

Which of the following might lead to the generation of several incorrect PCR products?

A recombinant DNA molecule is produced artificially and contains sequences ________.

Dideoxyribonucleotides (ddNTPs) used for DNA sequencing lack oxygen atoms at ________.

Which of the following techniques is the best choice for amplifying DNA?

Which of the following techniques is most commonly used to separate and analyze DNA by size?

The Biotechnology Century and Its Workforce

An Introduction to Genes and Genomes

Proteins As Products

Microbial Biotechnology

Plant Biotechnology

Animal Biotechnology

Dna Fingerprinting and Forensic Analysis

Bioremediation

Aquatic Biotechnology

Medical Biotechnology

Biotechnology Regulations

Ethics and Biotechnology

Filters