Question 1

Which of the following classification methods relies on the morphology of organisms?

Accepted Answer

A)   phage typing
B)   physical characteristics
C)   biochemical tests
D)   analysis of nucleic acids
E)   serological tests B

Question 2

A cell's G + C ratio is associated with which of the following classification methods?

Accepted Answer

A)   phage typing
B)   biochemical tests
C)   physical characteristics
D)   analysis of nucleic acids
E)   serological tests D

Question 3

In the Gram stain procedure, iodine serves as a

Accepted Answer

A)   counterstain.
B)   decolorizing agent.
C)   mordant.
D)   primary stain.
E)   fixative. C

Question 4

All of the following are associated with different types of phase microscopes EXCEPT

Accepted Answer

A)   a dark-field stop.
B)   a phase plate.
C)   Nomarski microscopes.
D)   unstained specimens.
E)   prisms.

Question 5

Which of the following is(are) (a) magnifying lens(es)?

Accepted Answer

A)   objective
B)   ocular
C)   condenser
D)   dark-field stop
E)   both the objective and the ocular

Question 6

If you were trying to visualize flagella without staining, which microscope would you use?

Accepted Answer

A)   phase-contrast
B)   dark-field
C)   fluorescent
D)   confocal
E)   bright-field

Question 7

Light rays that are in phase reinforce each other and produce a brighter image.

Accepted Answer

A) True 
 B)False

Question 8

A(n) __________ microscope has a single ocular lens.

Accepted Answer

The answer of A(n) __________ microscope has a single ocular...

Question 9

The limit of resolution of the unaided human eye is about __________ μm. (Be sure your answer is a numeral.)

Accepted Answer

The answer of The limit of resolution of the unaided...

Question 10

The ability of a lens to gather light is referred to as its

Accepted Answer

A)   resolution.
B)   numerical aperture.
C)   refraction.
D)   contrast.
E)   magnification.

Question 11

Why are modern light microscopes better than the ones Leeuwenhoek used?

Accepted Answer

A)   Modern microscopes have a fivefold better resolution.
B)   Modern microscopes are compound instead of simple.
C)   Modern microscopes have lenses with smaller numerical apertures.
D)   Modern lenses are made of prisms.
E)   Modern microscopes are compound and have fivefold better resolution.

Question 12

The microscope preferred for viewing living specimens is the __________ microscope.

Accepted Answer

A)   bright-field
B)   phase-contrast
C)   scanning electron
D)   scanning tunneling
E)   transmission electron

Question 13

Compare and contrast the light microscope with the electron microscope.

Accepted Answer

The answer of Compare and contrast the light microscope with...

Question 14

All of the following are types of light microscopes EXCEPT

Accepted Answer

A)   fluorescent.
B)   confocal.
C)   phase-contrast.
D)   scanning tunneling.
E)   bright-field.

Question 15

You are a scientist studying the highly specific interactions of bacteriophages with their host cells when they first encounter the cell. Discuss what microscope(s) and preparation procedures you might use for this study.

Accepted Answer

The answer of You are a scientist studying the highly...

Question 16

A(n) __________ key is a guide that directs the user through a step-by-step process to identify a microbe.

Accepted Answer

The answer of A(n) __________ key is a guide that...

Question 17

A staining procedure that uses a single basic dye is called a(n) __________ stain.

Accepted Answer

The answer of A staining procedure that uses a single...

Question 18

Carl Woese and his colleagues proposed the __________, a taxon that contains multiple kingdoms.

Accepted Answer

The answer of Carl Woese and his colleagues proposed the...

Question 19

Coating a specimen with a heavy metal is a step in preparing it for __________ microscopy.

Accepted Answer

The answer of Coating a specimen with a heavy metal...

Question 20

A(n) __________ is a substance that binds to a dye and makes it less soluble.

Accepted Answer

The answer of A(n) __________ is a substance that binds...

Exam 4: Microscopy, Staining, and Classification