Next- Generation Sequencing Takes Advantage of the Concept of Sequencing-

Multiple Choice

Next- generation sequencing takes advantage of the concept of sequencing- by- synthesis.What is sequence- by- synthesis?

A) The use of PCR to incorporate ddNTPs,followed by electrophoresis.
B) The use of a multiwell system and microfluidics to detect incorporated nucleotides at each base.
C) The use of firefly luciferase and pyrophosphate to report on nucleotide incorporation.
D) The use of a single polymerase in a nanopore that reports on a single molecule of synthesized DNA.