Multiple Choice
Sanger sequencing is based on the order in which ddNTPs are added to a growing polynucleotide.Why are ddNTPs integral to the Sanger sequencing method?
A) They have a 3' hydroxyl that allows for extension of the polynucleotide.
B) They do not have a 2' hydroxyl,which does not allow the extension of the polynucleotide.
C) They do not have a 3' hydroxyl,which does not allow the extension of the polynucleotide.
D) They have a 2' hydroxyl that allows for extension of the polynucleotide.
Correct Answer:
Verified
Correct Answer:
Verified
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