Reaction of a Polypeptide,composed of 12 Amino Acids,with Carboxypeptidase a Releases

Multiple Choice

Reaction of a polypeptide,composed of 12 amino acids,with carboxypeptidase A releases Met (C-terminal amino acid) .The polypeptide undergoes partial hydrolysis to give 12 groups of peptides.Use the groups of overlapping amino acids to determine the proper sequence of this polypeptide.Note: Since these lists of peptides are separated by commas,they are not necessarily in the proper sequence. Ser,Lys,Trp-5.Met,Ala,Gly- 9.Lys,Ser
Gly,His,Ala-6.Ser,Lys,Val -10.Glu,His,Val
Glu,Val,Ser-7.Glu,His-11.Trp,Leu,Glu
Leu,Glu,Ser-8.Leu,Lys,Trp-12.Ala,Met

A) Met-Ala-Gly-His-Glu-Val-Ser-Lys-Trp-Leu-Glu-Ser
B) Met-Ala-Gly-Glu-His-Ser-Val-Lys-Trp-Leu-Glu-Ser
C) Ser-Glu-Leu-Trp-Lys-Ser-Val-Glu-His-Gly-Ala-Met
D) Ser-Lys-Leu-Trp-Lys-Ser-Val-His-Glu-Gly-Ala-Met