Fill in the Missing Blanks with Single Words

Essay

Fill in the missing blanks with single words.
Genome _____1______ means artificially introducing a specific change in the DNA sequence at a unique, pre-determined location within the genome of an ____2____ cell. The method relies on some form of recognition of specific sequences on both DNA strands at a locus that then allows an artificially introduced _____3____-stranded DNA break at this location. In response to the _____3____-stranded DNA break, DNA repair is carried out by the cell but after using non_____4_____ end joining DNA repair, errors can be made in the repair that can occasionally result in a desired specific DNA change. In one system genetically engineered ______5_____ ______6_____ nucleases are used in which a DNA is constructed to code for a specific sequence of ______5______ _____6______ and is then ligated to a DNA sequence that will specify a DNA ______7______ domain. A plasmid containing the resulting DNA construct can encode a ______5______ _____6______ nuclease when transfected into a cell. Using this technology, a pair of ______5______ _____6______ nucleases can be designed to bind to specific sequences on the opposite DNA strands at a desired unique position in the genome and the adjoining DNA cleavage domains work to produce the required ____3_____-strand DNA break.