Concerning Making Animal Models of Human Disease, Which, If Any

Multiple Choice

Concerning making animal models of human disease, which, if any, of the following statements is false?

A) Pronuclear microinjection is a general way of making a transgenic animal and involves microinjection of foreign DNA into a fertilized egg cell.
B) Pronuclear microinjection is best suited to modelling recessively inherited single gene disorders.
C) Gene targeting using embryonic stem cells depends on having well-characterized embryonic stem cell lines that can readily allow transmission through the germ line.
D) Gene targeting using embryonic stem cells in mice is a popular way of modelling disease phenotypes that result from a gain-of-function.