Which of the restriction nucleases listed below can potentially cleave a segment of cDNA that encodes the peptide KIGDACF?

\text { The Genetic Code }

\begin{array}{|l|l|l|l|l|}\hline & \text { U } & \text { C } & \text { A } & \text { G } \\\hline &\text { UUU Phe (F) } & \text { UCU Ser (S) } & \text { UAU Tyr (Y) } & \text { UGU Cys (C) } \\&\text { UUC - } & \text { UCC - } & \text { UAC - } & \text { UGC - } \\ \text { U } &\text { UUA Leu (L) } & \text { UCA -- } & \text { UAA Stop } & \text { UGA Stop } \\&\text { UUG - } & \text { UCG - } & \text { UAG Stop } & \text { UGG Trp (W) } \\\hline &\text { CUU Leu (L) } & \text { CCU Pro (P) } & \text { CAU His (H) } & \text { CGU Arg (R) } \\&\text { CUC - } & \text { CCC - } & \text { CAC - } & \text { CGC - } \\ \text { C}&\text { CUA - } & \text { CCA - } & \text { CAA Gin (Q) } & \text { CGA - } \\&\text { CUG }- & \text { CCG }- & \text { CAG }- & \text { CGG }- \\\hline &\text { AUU Ile (I) } & \text { ACU Thr (T) } & \text { AAU Asn (N) } & \text { AGU Ser (S) } \\&\text { AUC - } & \text { ACC - } & \text { AAC - } & \text { AGC - } \\ \text { A}&\text { AUA - } & \text { ACA - } & \text { AAA Lys (K) } & \text { AGA Arg (R) } \\&\text { AUG Met (M) } & \text { ACG - } & \text { AAG - } & \text { AGG - } \\\hline &\text { GUU Val (V) } & \text { GCU Ala (A) } & \text { GAU Asp (D) } & \text { GGU Gly (G) } \\&\text { GUC - } & \text { GCC - } & \text { GAC - } & \text { GGC - } \\ \text { G}&\text { GUA - } & \text { GCA - } & \text { GAA Glu (E) } & \text { GGA - } \\&\text { GUG - } & \text { GCG - } & \text { GAG - } & \text { GGG - } \\\hline\end{array}

Table 10-56

\begin{array}{cc}\underline{\text {Restriction Nuclease}}&\underline{\text {Recognition Sequence}}\\\text { EcoRI } & \text { GAATTC } \\ \text { HindIII } & \text { AAGCTT } \\ \text { Nsil } & \text { ATGCAT }\end{array}

You Have Purified DNA from Your Recently Deceased Goldfish

Multiple Choice