Multiple Choice
Why is an excess of normal deoxyribonucleoside triphosphate molecules (dNTPs) needed during dideoxy sequencing?
A) DNA polymerase uses the dNTPs to synthesize a DNA molecule complementary to the molecule being sequenced.
B) dNTPs are consumed as energy to fuel the sequencing reactions.
C) When dNTP levels are too low, there will be very few chain-termination events.
D) The dNTPs can hybridize to the fragment to be sequenced and serve as primers for DNA polymerase.
Correct Answer:
Verified
Correct Answer:
Verified
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