Some Plasmids from CDNA Libraries Can Have Defects Because of the Way

Essay

Some plasmids from cDNA libraries can have defects because of the way in which a cDNA library is constructed.For each dilemma (A to D) listed below, indicate which of the outcomes (1 to 4) you might encounter, and explain why.Outcomes may be used more than once. $\begin{array}{llcc}\underline{ \text { Dilemma }\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad} & \underline{ \text { Outcomes\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad} }\\ \text { (A) The mRNA corresponding to the clone you are } &\text { (1) The 5' part of the gene will be missing. } \\ \text { looking for was degraded at its 5' end by a } &\\ \text { nuclease.} &\\\\ \text {(B) The mRNA corresponding to the clone you are } & \text {(2) The \( 3^{\prime} \) part of the gene will be missing. }\\ \text { looking for was degraded at its \( 3^{\prime} \) end by a } &\\ \text { nuclease. } & \\ \\ \text { (C) The S'end of the reverse transcriptase product of } & \text {(3) An internal fragment of the gene will be missing }\\ \text { the gene you are trying to clone hybridizes to } &\\ \text { sequences in the middle of the gene. } &\\\\ \text {(D) The gene you are trying to clone has a long } & \text { (4) The gene will be missing from the library.}\\ \text {stretch of A's in the middle of the coding } &\\ \text { sequence. } &\\\end{array}$

Table 10-54