You Isolate a New Bacterium from Soil with a Revolutionary

Multiple Choice

You isolate a new bacterium from soil with a revolutionary new culture technique.You are interested in sequencing the genome of this organism using the whole-genome,or shotgun,sequencing approach.Of the following choices,what would be the best way to prepare the genomic DNA for sequencing?

A) digestion with HpaI (CCGG)
B) digestion with BamHI (GGATCC)
C) digestion with EcoRI (GAATTC)
D) digestion with NotI (GCGGCCGC)
E) digestion with EcoRI,BamHI,and HpaI