You Have Discovered a Gene That Appears to Be Crucial

Multiple Choice

You have discovered a gene that appears to be crucial to the development of the nervous system in C.elegans.Which of the following techniques would allow you to investigate the transcriptional regulation and expression of this gene in vivo?

A) DNA cloning
B) restriction endonuclease mapping
C) replacing one copy of the gene with a reporter gene,such as GFP or lacZ
D) transcriptional activator-like effector nucleases (TALENs) targeted gene disruption
E) RNAi gene knockdown