To Generate a Knockin Mouse, a Scientist Introduces to ES

Multiple Choice

To generate a knockin mouse, a scientist introduces to ES cells a construct with a mutant exon and within an adjacent intron, loxP sites flanking a neomycin resistance gene.What event will occur next?

A) Homologous recombination between the mutant DNA and the corresponding gene in the mouse genome.
B) Recombination between the loxP sites.
C) Transplantation of cells into host blastocyst.
D) Crossing of heterozygous mice to a Cre-expressing strain.