Of These Steps, Which One Occurs Earliest in the Process

Multiple Choice

Of these steps, which one occurs earliest in the process of producing recombinant DNA?

A) Human DNA fragments are mixed with the cut plasmids.
B) Restriction enzymes are used to isolate the gene of interest and to cut the plasmid DNA.
C) Recombinant plasmids are mixed with bacteria.
D) Bacteria carrying recombinant plasmids are cloned.