You Have Determined That a Bacterial Strain You Are Working

Essay

You have determined that a bacterial strain you are working with contains a single type of plasmid. You isolate the plasmid DNA and digest separate portions of it with each of two different restriction enzymes, BamHI and HpaI, and also perform a double digest using both enzymes. You then fractionate the enzyme digests on an agarose gel and stain the gel with ethidium bromide to visualize the restriction fragment patterns. Your results are shown below. Size markers (in nucleotide base pairs) are indicated at the left side of the gel. Using these data, construct a possible restriction map for the plasmid.