Use the Following to Answer Questions

Multiple Choice

Use the following to answer questions
A student carries out PCR using the following steps:
Step 1: 94°C for 1 minute
Step 2: 60°C for 30 seconds
Step 3: 72°C for 30 seconds
-After subjecting his PCR reaction to gel electrophoresis, the student sees no PCR product on the gel. What error(s) might he have made? (Select all that apply.)

A) He carried out step 1 at too low a temperature.
B) He carried out step 1 at too high a temperature.
C) He carried out step 2 at too low a temperature.
D) He carried out step 2 at too high a temperature.
E) He carried out step 3 for too short a time.